Gap junction intercellular communication channels permit the exchange of small regulatory molecules and ions between neighbouring cells and coordinate cellular activity in diverse tissue and organ systems. These channels have short half‐lives and complex assembly and degradation pathways. Much of the recent work elucidating gap junction biogenesis has featured the use of connexins (Cx), the constituent proteins of gap junctions, tagged with reporter proteins such as Green Fluorescent Protein (GFP) and has illuminated the dynamics of channel assembly in live cells (...) by high‐resolution time‐lapse microscopy. With some studies, however, there are potential short‐comings associated with the GFP chimeric protein technologies. A recent report by Gaietta et al., has highlighted the use of recombinant proteins with tetracysteine tags attached to the carboxyl terminus of Cx43, which differentially labels ‘old’ and ‘new’ connexins thus opening up new avenues for studying temporal and spatial localisation of proteins and in situ trafficking events.1 BioEssays 24:876–880, 2002. © 2002 Wiley Periodicals, Inc. (shrink)

LetL(K) denote the lattice (ordered by inclusion) of quasivarieties contained in a quasivarietyK and letD 2 denote the variety of distributive (0, 1)-lattices with 2 additional nullary operations. In the present paperL(D 2) is described. As a consequence, ifM+N stands for the lattice join of the quasivarietiesM andN, then minimal quasivarietiesV 0,V 1, andV 2 are given each of which is generated by a 2-element algebra and such that the latticeL(V 0+V1), though infinite, still admits an easy and nice description (...) (see Figure 2) while the latticeL(V 0+V1+V2), because of its intricate inner structure, does not. In particular, it is shown thatL(V 0+V1+V2) contains as a sublattice the ideal lattice of a free lattice with free generators. Each of the quasivarietiesV 0,V 1, andV 2 is generated by a 2-element algebra inD 2. (shrink)

The influence of Goethe on Wittgenstein is just beginning to be appreciated. Hacker and Baker, Westphal, Monk, and Haller have all drawn attention to significant affinities between the two men's work, and the number of explicit citations of Goethe in Wittgenstein's texts supports the idea that we are not dealing simply with a matter of deeplying similarities of aim and method, but of direct and major influence. These scholarly developments are encouraging because they help to place Wittgenstein's work within an (...) important tradition of German letters which goes far beyond his contemporaries and immediate forebears in Vienna; and they show that Wittgenstein's profound interest in literature and music is ceasing to be merely a matter of biographical anecdote, and is being used to illuminate some of the most central areas of his work. (shrink)

This paper gives a simple, elegant statement of the condensed detachment rule that is independent of most general unifiers and proves that this is equivalent to the longer, more usual, formulation.

THE first Epode provides no clear indication of date. We learn only that Maecenas is about to join Octavian on a dangerous expedition and has suggested that Horace should not accompany him, while Horace retorts that he will be unable to enjoy himself in the absence of his patron and would be ready to follow him to the ends of the earth, whatever the danger, in the hope of earning his gratitude. The Epodes were published about 30 B.C. and, perhaps (...) for that reason, the scholiast Pseudo-Acron confidently assigns the poem to the period immediately before the battle of Actium with the comment: ‘Maecenatem prosequitur euntem ad bellum navale cum Augusto adversum Antonium et Cleopatram.’ It is curious that in referring to the activities of Octavian in 31 the author uses the title Augustus, which was not conferred on him before 27,: but the substance of his comment accords with the known facts. In the spring of 31 Octavian asked all Romans of influence to meet him at Brundisium, thereby to demonstrate their willingness to take part in the war against Antony and Cleopatra, and more than seven hundred senators are known to have offered their services. (shrink)